Jan De Winne concurrently studied flute and music theory at the Conservatory and University of Ghent, and subsequently studied traverso under Barthold Kuijken at the Brussels Conservatory. Between 1990 and 2005 he played with various ensembles in Belgium and abroad, such as the Orchestre des Champs Elysées, Collegium Vocale Gent and the Wiener Akademie. In 1988 he founded Il Gardellino with Marcel Ponseele, with which he gave concerts throughout Europe as well as in South and central America, Japan, Australia and Russia.
His interest in old instruments also led him to make his own copies of 18th and 19th century traversos. Jan de Winne teaches traverso and historical performance practice at the Conservatoire National Supérieur de Musique et de Danse in Paris and historical chamber music at the Royal Brussels Conservatory. He has recorded both as a soloist and as a chamber musician.